Highly Reactive Abasic DNA
نویسندگان
چکیده
منابع مشابه
The chemical stability of abasic RNA compared to abasic DNA
We describe the synthesis of an abasic RNA phosphoramidite carrying a photocleavable 1-(2-nitrophenyl)ethyl (NPE) group at the anomeric center and a triisopropylsilyloxymethyl (TOM) group as 2'-O-protecting group together with the analogous DNA and the 2'-OMe RNA abasic building blocks. These units were incorporated into RNA-, 2'-OMe-RNA- and DNA for the purpose of studying their chemical stabi...
متن کاملProteomic approach to identification of proteins reactive for abasic sites in DNA.
Apurinic/apyrimidinic (AP) sites, a prominent type of DNA damage, are repaired through the base excision repair mechanism in both prokaryotes and eukaryotes and may interfere with many other cellular processes. A full repertoire of AP site-binding proteins in cells is presently unknown, preventing reliable assessment of harm inflicted by these ubiquitous lesions and of their involvement in the ...
متن کاملDissociative electron attachment to abasic DNA.
Thin films of the short single DNA strand, GCAT, in which one of the bases has been removed were bombarded with 3 to 15 eV electrons. The yield functions of the H(-), O(-) and OH(-) ions desorbed from these films exhibit a broad peak near 9 eV, which is attributed to dissociative electron attachment to the basic molecules. Whereas removal of any one of the bases considerably decreases N-glycosi...
متن کاملTranslesion replication of abasic sites in DNA
Abasic sites (AP sites) arise very frequently in DNA and represent a common intermediate in numerous DNA repair systems. However, an unrepaired AP site poses a serious problem to the advancement of DNA polymerases (Pols) because the modified base has lost its coding capacity. Nevertheless, many Pols, either replicatives or specialized, can replicate AP sites, at least in vitro, with variable ef...
متن کاملSynthesis of Cross-Linked DNA Containing Oxidized Abasic Site Analogues
DNA interstrand cross-links are an important family of DNA damage that block replication and transcription. Recently, it was discovered that oxidized abasic sites react with the opposing strand of DNA to produce interstrand cross-links. Some of the cross-links between 2'-deoxyadenosine and the oxidized abasic sites, 5'-(2-phosphoryl-1,4-dioxobutane) (DOB) and the C4-hydroxylated abasic site (C4...
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ژورنال
عنوان ژورنال: Science
سال: 2014
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.343.6175.1058-c